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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
Detection of animal viruses using nucleic acid sequence-based amplification (NASBA)
L T Lau1, Y W W Fung, A C H Yu
1Hong Kong DNA Chips Ltd, Hang Tung Resources Centre, Shau Kei Wan, China.
Developments in Biologicals
|October 25, 2006
Summary
Efficient molecular diagnostics, like nucleic acid sequence-based amplification (NASBA), are crucial for early detection of animal viruses. This technology aids in prompt containment measures, preventing widespread disease outbreaks.
Area of Science:
- Veterinary Virology
- Molecular Diagnostics
- Infectious Disease Control
Background:
- Avian influenza outbreaks highlight the critical need for effective disease prevention strategies.
- Early detection of emerging viruses is essential for successful containment and minimizing disease spread.
Purpose of the Study:
- To review and describe molecular diagnostic technologies for detecting animal viruses.
- To emphasize the utility of nucleic acid sequence-based amplification (NASBA) in disease surveillance.
Main Methods:
- Review of molecular diagnostic techniques, with a focus on NASBA.
- Application of NASBA for detecting viruses like avian influenza, foot-and-mouth disease, and Newcastle disease.
Main Results:
- NASBA demonstrates high sensitivity, specificity, and accuracy in virus detection.
- NASBA provides rapid availability of results, crucial for timely interventions.
Conclusions:
- Nucleic acid sequence-based amplification (NASBA) is a highly applicable molecular diagnostic tool.
- NASBA can significantly enhance disease surveillance and control programs for animal diseases.
