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Published on: November 11, 2022
[Fingerprints of Rhizoma Coptidis from Shizhu by HPLC]
Xiao-ping Huang1, Long-yun Li, Xian-you Qu
1Chongqing Academy of Chinese Materia Medica, China.
Objective:
To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing.
Methods:
The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm.
Results:
The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine.
Conclusion:
The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality.