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[Optimizing the extracting technique of ampelopsin from Ampelopsis cantoniensis Planch by a uniform design method]
Zhi-feng He1, Sa Zeng, Juan-juan Hou
1School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China.
Objective:
To optimize the preparation of ampelopsin from Ampelopsis Cantoniensis Planch.
Methods:
The extraction and purification process was studied by the uniform design with the extract of ampelopsin content and purity as markers. The facters which influence the extraction and the purification of ampelopsin content were studied by uniform design.
Results:
The optimum extraction and purification process: the concentration for alcohol was 90%, and refluxing quartic, 1.5 h each time; extraction by petroleum ether quintic, the mount of active carbon was 1 g/100 g of the medicine material, and recrystaling thrice.
Conclusion:
This extraction process has higher yield of ampelopsin and is available for production.
