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Published on: August 14, 2019
HPLC-MS analysis of the riboflavin crude product of semisynthesis
Jianyu Guo1, Yan Lu, Haiou Dong
1Department of Chemistry, Shanghai Normal University, Shanghai 200234, China. luuyann@sit.edu.cn
Abstract:
An accurate and simple analysis method by high-performance liquid chromatography-mass spectrometry for the riboflavin crude product of semisynthesis is established in this study. In this method, a C(18) column is used, and the mobile phase is methanol-water. Under the optimal analysis conditions established in this study, all components in the riboflavin crude product are well separated and identified, respectively. The external standard method is used for the quantitative analysis, and the results are proven to be accurate.
