Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-dependent changes in donor brain death related processes.

T A Schuurs1, A M Morariu, P J Ottens

  • 1Department of Surgery, University Medical Center Groningen, Groningen, The Netherlands. t.a.schuurs@chir.umcg.nl

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
|October 26, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomarkers of standard criteria and marginal donor lungs during ex vivo lung perfusion: A comparative study.

JHLT open·2026
Same author

Symptom Burden and Patient-Reported Outcomes in Kidney Transplant Recipients: Results From the TransplantLines Biobank and Cohort Studies.

Kidney medicine·2026
Same author

Evaluation of donor's hormonal profile according to sex and age.

Frontiers in physiology·2025
Same author

Iron chelation prior to static cold storage decreases lipid peroxidation and improves gluconeogenesis during NMP in isolated porcine kidneys.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Does ACR TI-RADS increase efficacy of thyroid ultrasounds?

Clinical imaging·2025
Same author

Variability in perfusion conditions and set-up parameters used in ex vivo human placenta models: A literature review.

Placenta·2024
Same journal

Paclitaxel-Coated Balloon Therapy for Refractory Ureteral Stenosis in a Kidney Transplant Recipient.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same journal

Prophylactic Omentopexy to Prevent Proximal Duodenal Staple Line Leak in Whole Organ Pancreas Transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same journal

Intraorgan and Targeted Nanodelivery in Organ Transplantation of Non-Human Primates.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same journal

Immunogenomic landscape of the T cell repertoire after human lung transplantation and its clinical relevance.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same journal

Reversing pregnancy-induced B cell sensitization to facilitate the induction of transplant tolerance in post-partum recipients.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same journal

Total Robotic Liver Transplant Using Whole Graft with Variant Donor Arterial and Biliary Anatomy.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
See all related articles

Brain death rapidly triggers inflammation and protective gene expression in donor kidneys. Early chemokine increases, not hypoxia, initiate this cascade, impacting kidney transplant outcomes.

Area of Science:

  • Nephrology
  • Transplantation Immunology
  • Molecular Biology

Background:

  • Brain death (BD) negatively impacts kidney graft function and survival.
  • Kidney donor brains exhibit inflammation, coagulation, and cytoprotective gene activation.
  • Understanding the temporal progression of these BD-related renal processes is crucial.

Purpose of the Study:

  • To investigate the time-course progression of renal processes following brain death.
  • To identify early molecular and cellular changes in donor kidneys after BD.
  • To determine the initial triggers of the inflammatory cascade in BD kidneys.

Main Methods:

  • Time-course analysis of gene expression (E-selectin, MCP-1, IL-6, HO-1, HSP70, NF-kappaB, Egr-1) in kidneys post-BD.
  • Assessment of infiltrating granulocytes and reactive oxygen species formation.

Related Experiment Videos

  • Measurement of serum cytokines (MCP-1, KC-protein) at various time points after BD.
  • Comparison with sham-operated control animals.

    • Main Results:

    • Massive upregulation of proinflammatory genes (E-selectin, MCP-1, IL-6) and inducers (NF-kappaB, Egr-1) within 0.5 hours post-BD.
    • Increased granulocyte infiltration observed from 0.5 hours onwards.
    • Early induction of protective genes (HO-1, HSP70) within 0.5 hours.
    • Significant elevation of MCP-1 and KC-protein in serum starting at 0.5 hours.
    • Reactive oxygen species formation detected only in later BD phases.

    Conclusions:

    • Brain death rapidly induces both proinflammatory and stress-induced protective responses in donor kidneys.
    • Early changes during BD induction, not hypoxia, appear to trigger these processes.
    • Elevated systemic chemokines (MCP-1, KC) likely initiate the inflammatory cascade in brain-dead donor kidneys.