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Updated: Jul 19, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
[Determination of diltiazem hydrochloride injection by high-performance liquid chromatography]
Ling Tang1, Ting Zhang, Xin-zhong Li
1Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410013, China.
Objective:
To establish an high-performance liquid chromatography (HPLC) method for the determination of diltiazem hydrochloride injection.
Methods:
The assay was conducted on a NUCLEODUR C18 column with buffer solution (pH6.2)-acetonitrile-methanol (40:30:30) as the mobile phase and the detection wavelength was 240 nm.
Results:
Diltiazem hydrochloride and relevant substances could be separated under the condition. The linear concentration range of diltiazem hydrochloride was 4 - 200mg/L with the recovery of 100.22% (RSD = 0.37%).
Conclusion:
The method may be used for the determination of diltiazem hydrochloride injection.
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