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Updated: Jun 10, 2025

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CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
Published on: November 1, 2024
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Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology
Tingyu Gong1, Dandan Liu2, Xiaochen Wang2
1Shulan International Medical College, Zhejiang Shuren University, Hangzhou, 310015, China.
Stem Cell Research
|October 19, 2024
Abstract:
Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype.

