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Updated: Jul 19, 2026
07:29
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
High density lipoprotein subfractions: isolation, composition, and their duplicitous role in oxidation
Peter A C McPherson1, Ian S Young, Bronac McKibben
1Centre for Clinical and Population Sciences, Nutrition and Metabolism Group, Queen's University, Belfast, United Kingdom.
Journal of Lipid Research
|October 27, 2006
Summary
This study introduces a faster method for subfractionating high-density lipoprotein (HDL) and reveals its dual role in oxidation, acting as both a prooxidant and antioxidant in different contexts.
Area of Science:
- Lipid biochemistry
- Cardiovascular research
- Atherosclerosis
Background:
- Plasma high-density lipoproteins (HDL) are crucial for cholesterol transport and exist as HDL(2) and HDL(3) subfractions.
- Current HDL subfractionation methods are time-consuming, limiting large-scale studies.
Purpose of the Study:
- To develop a rapid method for HDL subfractionation.
- To investigate the role of HDL subfractions in lipoprotein oxidation and atherosclerosis.
Main Methods:
- Developed a novel 6-hour method for HDL subfractionation from plasma.
- Assessed lipid and protein components of isolated HDL subfractions.
- Evaluated HDL subfractions' susceptibility to oxidation with VLDL and LDL.
Main Results:
- HDL exhibits a prooxidant role in VLDL oxidation via cholesteryl ester transfer protein (CETP).
- HDL acts as an antioxidant in LDL oxidation.
- LCAT acts as a prooxidant in VLDL oxidation but an antioxidant in LDL oxidation.
Conclusions:
- HDL plays a complex, dual role in lipoprotein oxidation.
- HDL's prooxidant and antioxidant activities, influenced by CETP and LCAT, may contribute to atherosclerosis pathogenesis.
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