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Updated: Aug 8, 2026

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
Published on: January 1, 2016
Expression of intermediate filament proteins in the adult human cochlea
L J Bauwens1, J E Veldman, H Bouman
1Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.
Abstract:
The immunohistochemical localization of intermediate filament proteins was studied in frozen sections of chemically fixed, nondecalcified adult human cochleas. Cytokeratins were found in all epithelial cells lining the cochlear duct (including most supporting cells of the organ of Corti) but were absent in the hair cells. Neurofilament proteins were present in the nerve endings at the hair cells, in the neural bundles, and in the ganglion cells. Vimentin staining occurred in most of the supporting structures and was roughly complementary to the regions showing cytokeratin staining and neurofilament staining. However, the region of the spiral prominence and outer sulcus, as well as the pillar cells and Deiters' cells in the organ of Corti, showed coexpression of vimentin and cytokeratins. No definite immunostaining was observed with antibodies to desmin and glial fibrillary acidic protein.
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