Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression profiling in a mouse model for African trypanosomiasis.

S Kierstein1, H Noyes, J Naessens

  • 1International Livestock Research Institute, Nairobi, Kenya. skierstein@mac.com

Genes and Immunity
|October 27, 2006
PubMed
Summary

This study reveals key gene expression differences between susceptible and tolerant mice infected with Trypanosoma congolense. Susceptibility is linked to higher expression of inflammatory genes, while tolerance is associated with immune response and metabolism genes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgical management of metastatic cutaneous Crohn's disease: a case series from a tertiary centre in the United Kingdom.

Techniques in coloproctology·2026
Same author

Diagnosis of <i>Mycoplasma columborale</i> in a fledgling kererū (<i>Hemiphaga novaeseelandiae</i>).

New Zealand veterinary journal·2024
Same author

A blockchain-based framework to support pharmacogenetic data sharing.

The pharmacogenomics journal·2022
Same author

Learning to implement Smart Healthy Age-Friendly Environments.

Translational medicine @ UniSa·2021
Same author

Comparing the accuracy of lay diagnosis of childhood malaria and pneumonia with that of the revised IMCI guidelines in Nigeria.

Journal of public health (Oxford, England)·2020
Same author

International comparison of civilian violent deaths: a public health approach to reduce gun-related deaths in US youth.

Public health·2019

Area of Science:

  • Immunology
  • Genomics
  • Parasitology

Background:

  • Trypanosoma congolense infection causes significant disease in susceptible mouse strains.
  • Understanding the genetic basis of host response is crucial for developing effective treatments.

Purpose of the Study:

  • To identify novel molecular mechanisms and pathways involved in the host response to Trypanosoma congolense infection.
  • To compare gene expression profiles between susceptible (A/J) and tolerant (C57BL/6) mice.

Main Methods:

  • Large-scale gene expression analysis using microarrays.
  • Monitoring of parasitemia and anemia kinetics in infected mice.
  • RNA extraction from liver tissue at multiple time points post-infection.

Main Results:

Related Experiment Videos

  • Identified 64 differentially expressed genes between mouse strains in uninfected animals, with nine unaffected by infection.
  • Susceptible mice showed higher expression of chemokines, complement components, and interferon receptor alpha.
  • Tolerant mice exhibited higher expression of immune response, metabolism, and protease genes.

Conclusions:

  • Host susceptibility to Trypanosoma congolense is associated with specific gene expression patterns, particularly involving inflammatory pathways.
  • Gene expression profiling provides insights into previously overlooked mechanisms of host-parasite interactions.