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Updated: Jan 18, 2026

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Imaging single-channel calcium microdomains.

Angelo Demuro¹, Ian Parker

¹Department of Neurobiology and Behavior, University of California, Irvine, CA 92697-4550, USA.

|October 28, 2006

Summary

This summary is machine-generated.

New optical imaging visualizes calcium (Ca2+) microdomains at the single-channel level. This "optical patch-clamp" method offers detailed insights into ion channel function.

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Last Updated: Jan 18, 2026

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Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Area of Science:

Biophysics
Cell Biology
Neuroscience

Background:

Cytosolic calcium signals are built from Ca(2+) microdomains near open ion channels.
Traditional methods like patch-clamp recordings are limited in scale and scope.

Purpose of the Study:

To review advances in single-channel Ca(2+) imaging.
To highlight Total Internal Reflection Fluorescence Microscopy (TIRFM) for optical patch-clamp recording.

Main Methods:

Utilizing TIRFM for high-resolution imaging of Ca(2+) transients.
Developing single channel calcium fluorescence transients (SCCaFTs) as a readout.
Applying the technique to voltage- and ligand-gated plasmalemmal ion channels.

Main Results:

SCCaFTs provide insights into channel properties previously requiring electrophysiology.
TIRFM enables massively parallel, simultaneous imaging of hundreds of channels.
Millisecond resolution of gating kinetics and sub-micron spatial resolution are achieved.

Conclusions:

Optical patch-clamp recording with TIRFM is a powerful, versatile tool.
This method significantly advances the study of ion channel function and Ca(2+) signaling.
Applicable to diverse membrane channels with partial Ca(2+) permeability.