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Surface-plasmon microscopy with a two-piece solid immersion lens: bright and dark fields.

Jing Zhang¹, Mark C Pitter, Shugang Liu

¹Applied Optics Group, School of Electronic Engineering, University of Nottingham, UK. eexjz@nottingham.ac.uk

|October 28, 2006

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This study introduces advanced surface-plasmon imaging techniques using a novel solid immersion lens. These methods achieve high-contrast, high-resolution imaging of protein monolayers in various environments.

Area of Science:

Optics and Photonics
Surface Science
Biophysics

Background:

Surface-plasmon imaging offers potential for high-resolution microscopy.
Existing techniques face limitations in contrast and sample manipulation.
Need for improved imaging methods for biological samples like protein monolayers.

Purpose of the Study:

To develop and demonstrate bright-field and dark-field surface-plasmon imaging.
To introduce a modified solid immersion lens for enhanced imaging.
To present a theoretical model for understanding imaging mechanisms.

Main Methods:

Utilized a modified solid immersion lens in an epi-illumination configuration.
Employed a commercial objective with moderate numerical aperture (NA).

Related Experiment Videos

Developed a two-part solid immersion lens for sample mobility.

Main Results:

Achieved excellent contrast and resolution in surface-plasmon imaging.
Successfully imaged protein monolayers in both air and aqueous environments.
Demonstrated that the two-part lens maintains image quality during sample movement.

Conclusions:

The developed surface-plasmon imaging approach provides superior image quality.
The modified solid immersion lens design facilitates sample manipulation.
A Green's function model effectively explains the imaging principles.