Related Experiment Video
Updated: Jul 19, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
Published on: October 28, 2025
Chromatic confocal spectral interferometry
Evangelos Papastathopoulos1, Klaus Körner, Wolfgang Osten
1Institut für Technische Optik, Universität Stuttgart, Germany. papastav@ito.uni-stuttgart.de
Chromatic confocal spectral interferometry (CCSI) is a new topography measurement technique. It combines spectral interferometry and chromatic confocal microscopy for single-shot, high-NA white-light detection and depth positioning.
Area of Science:
- Optics and Photonics
- Metrology
- Microscopy
Background:
- Topography measurements require precise depth sensing.
- Existing methods like spectral interferometry and chromatic confocal microscopy have limitations in range or resolution.
- A hybrid approach could overcome these limitations.
Purpose of the Study:
- To introduce and validate Chromatic Confocal Spectral Interferometry (CCSI) as a novel topography measurement technique.
- To demonstrate CCSI's capability for single-shot, high numerical aperture (NA) white-light interferometric detection.
- To show CCSI's ability to retrieve object depth position using interferometric signal phase.
Main Methods:
- Combining spectral interferometry and chromatic confocal microscopy.
- Utilizing a confocally filtered and chromatically dispersed focus for detection.
- Acquiring interferometric signals and analyzing their phase (modulation frequency) for depth retrieval.
Main Results:
- CCSI enables white-light interferometric detection with high NA in a single shot.
- The method successfully retrieves the depth position of reflecting or scattering objects.
- The depth range of the sensor is decoupled from the objective's NA due to the dispersed focus.
Conclusions:
- CCSI is a pioneering interferometric method for topography measurements.
- This technique offers a unique combination of high NA detection and depth positioning.
- CCSI advances metrology by decoupling sensor depth range from objective NA.
Related Concept Videos
IR Spectrometers
Confocal Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...

