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Updated: Jul 19, 2026

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model
Published on: June 18, 2021
The inflammation in the gut after experimental subarachnoid hemorrhage
Meng-Liang Zhou1, Lin Zhu, Jian Wang
1Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu Province, China.
Background:
Gastrointestinal dysfunction could be frequently observed in the patients suffering from SAH. This study test the hypothesis that experimental SAH could induce histopathological changes and inflammatory response associating with NF-kappaB activation pathway in the gut.
Materials And Methods:
A total of 17 rabbits were randomly divided into two groups: control group (n = 8) and SAH group (n = 9). In the SAH group, the animals were subjected to experimental SAH according to the "two-hemorrhage" method. The histopathological study was performed to detect the intestinal mucosal morphological changes and immunohistochemical study was used to detect the TNF-alpha and ICAM-1 expressions. NF-kappaB binding activity was measured using the electrophoretic mobility shift assay.
Results:
It was demonstrated that some damage changes and leukocytes infiltration occurred in the intestinal mucosa after SAH. More positive cells for TNF-alpha and ICAM-1 were observed in the SAH group. The NF-kappaB binding activity in the intestines was significantly increased in the SAH group (P < 0.01).
Conclusions:
The results of the present study suggest that SAH in the rabbits could induce NF-kappaB and proinflammatory cytokines activation in the intestine, which is associated with morphological changes.
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