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Targeting vector construction by yeast artificial chromosome modification.

Peter J Murray1

  • 1Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 31, 2006
PubMed
Summary
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Mouse yeast artificial chromosomes (YACs) offer a robust platform for creating gene targeting vectors when other methods fail. This procedure details how to modify YACs for diverse targeting vector applications.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Yeast artificial chromosomes (YACs) are valuable for manipulating large DNA fragments.
  • Traditional methods for generating targeting arms can be challenging with unstable DNA or when PCR amplification is unsuccessful.

Purpose of the Study:

  • To present a straightforward protocol for modifying mouse YACs.
  • To enable the production of versatile targeting vectors from YAC constructs.

Main Methods:

  • Modification of existing yeast artificial chromosomes (YACs).
  • Utilizing YACs as a platform for generating DNA constructs.

Main Results:

  • Successful modification of YACs to create targeting vectors.

Related Experiment Videos

  • Production of targeting vectors suitable for various applications.

    • Conclusions:

    • This method provides a reliable alternative for generating targeting vectors.
    • Mouse YACs serve as adaptable platforms for genetic engineering and vector construction.