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Related Experiment Videos

Methods developed for SELEX.

Subash Chandra Bose Gopinath1

  • 1Functional Nucleic Acids Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. gopi-subashchandrabose@aist.go.jp

Analytical and Bioanalytical Chemistry
|October 31, 2006
PubMed
Summary

Systematic evolution of ligands by exponential enrichment (SELEX) purifies high-affinity nucleic acid aptamers. This review examines separation techniques crucial for advancing aptamer development against diverse molecular targets.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • SELEX is an iterative process for isolating nucleic acid ligands with high target affinity.
  • Aptamer technology has expanded SELEX applications to various molecules, regardless of size.
  • The efficiency of SELEX relies heavily on effective separation methods.

Purpose of the Study:

  • To review and categorize the separation methods employed in SELEX processes.
  • To highlight advancements in SELEX separation techniques.
  • To provide a resource for researchers developing aptamers.

Main Methods:

  • Literature review of SELEX-related publications.
  • Categorization of separation techniques based on principles (e.g., affinity, size, charge).

Related Experiment Videos

  • Analysis of the advantages and disadvantages of different separation strategies.

    • Main Results:

    • Identification of diverse separation methods used in SELEX, including affinity chromatography, filtration, and electrophoresis.
    • Discussion of how specific separation techniques impact aptamer selection efficiency and specificity.
    • Overview of modified SELEX protocols incorporating novel separation strategies.

    Conclusions:

    • Effective separation is critical for successful aptamer selection via SELEX.
    • Ongoing innovation in separation technologies continues to enhance SELEX capabilities.
    • This review consolidates knowledge on SELEX separation, aiding future aptamer discovery.