Related Experiment Video
Updated: Jul 19, 2026
09:13
Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
Published on: May 12, 2023
Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast
Christopher J Penkett1, Zoë E Birtle, Jürg Bähler
1Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Cambridge CB10 1HH, UK.
Yeast (Chichester, England)
|October 31, 2006
Summary
This study introduces Pombe PCR Primer Programs (PPPP), a web tool automating primer design for yeast gene manipulation. A searchable database of microarray primers for Schizosaccharomyces pombe is also provided for broader applications.
Area of Science:
- Molecular Biology
- Genetics
- Bioinformatics
Background:
- PCR-based gene targeting is widely used for yeast gene manipulation.
- Manual primer design for these techniques is often time-consuming and prone to errors.
Purpose of the Study:
- To develop an automated tool for simplifying primer selection in Schizosaccharomyces pombe.
- To create a searchable database of primers for Schizosaccharomyces pombe microarrays.
Main Methods:
- Development of a web-based tool (Pombe PCR Primer Programs - PPPP) for automated primer design.
- Creation of a searchable Microarray Primer Database containing primer and PCR product information.
Main Results:
- The PPPP tool automates primer design for gene deletion, tagging, and regulated expression in S. pombe.
- The Microarray Primer Database provides sequences and information for S. pombe microarray construction and other PCR applications.
Conclusions:
- Automated tools like PPPP streamline genetic manipulation in yeast.
- The Microarray Primer Database offers valuable resources for S. pombe research, including quantitative PCR.
