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Published on: November 7, 2018
Hepatitis B virus genotypes in chronic liver disease patients from New Delhi, India
Saket Chattopadhyay1, Bhudev Chandra Das, Premashis Kar
1Hepatitis Laboratory, Department of Medicine, Maulana Azad Medical College, D-II/M-2755, Netaji Nagar, New Delhi 110023, India. saketchats@yahoo.com
Insights
Hepatitis B virus (HBV) genotypes A and D were identified in New Delhi, India, with genotype D being dominant in chronic liver disease patients. No significant clinical differences were observed between patients with HBV genotype A and D.
Area of Science:
- Hepatology
- Virology
- Genetics
Background:
- Hepatitis B virus (HBV) infection is a global health concern.
- HBV genotypes vary geographically and can influence disease progression.
- Understanding genotype distribution is crucial for managing chronic liver disease.
Purpose of the Study:
- To investigate the prevalence of HBV genotypes in patients with chronic liver disease (CLDB) in New Delhi, India.
- To determine the association between HBV genotypes and disease outcomes.
Main Methods:
- Sera from 100 CLDB patients were analyzed for HBV genotypes.
- Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Type-specific primers-based PCR (TSP-PCR) were employed.
- The surface (S) gene of HBV was targeted for genotyping.
Main Results:
- Only HBV genotypes A and D were detected in the study population.
- Genotype D was the predominant genotype (84%), found across all CLDB categories (chronic hepatitis B, cirrhosis, hepatocellular carcinoma).
- Genotype A was present in 16% of cases, and mixed infection (A+D) in 3%.
Conclusions:
- HBV genotypes A and D are the sole types circulating in CLDB patients in New Delhi.
- Genotype D is significantly more prevalent than genotype A.
- No significant clinical or biochemical differences were noted between patients infected with HBV genotype A and genotype D.
Aim:
To study the Hepatitis B virus (HBV) genotypes and their effect on the progression and outcome in patients with chronic liver diseases from New Delhi, India.
Methods:
Sera from 100 HBV-related chronic liver disease (CLDB) cases were tested for HBV genotype using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Type-specific primers-based PCR (TSP-PCR) targeting to the surface (S) gene encoding hepatitis B surface antigen.
Results:
Only genotypes A and D were present and genotype D was dominant. Genotype D was present in all CLDB patient categories. The genotype distribution for the 100 patients with CLDB was as follows: genotype A, 16/100 (16%) (7/40- 17% chronic hepatitis B (CHB); 8/47, 17%, HBV-related cirrhosis (CRB); 1/13, 7.6%, HBV-related hepatocellular carcinoma (HCCB); genotype D- 84/100 (84%) (32/40- 80% CHB; 38/47- 81%, CRB; 11/13, 85%, HCCB); genotype A+D, 3/100 (3%) (1/40- 3% CHB; 1/47- 2%, CRB; 1/13, 7.6%, HCCB); C, 0; B, 0; E, 0; F, 0; G 0, H 0; (P<0.01, genotype D vs A).
Conclusion:
Only HBV genotypes A and D were present in patients with CLDB from New Delhi, India. Compared with genotype D, genotype A patients had no significant clinical or biochemical differences (P>0.05). Mixed infection with genotype A and D were seen in 3% of the cases. Genotype D was the dominant genotype prevalent in all patient categories.
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