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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
Published on: March 25, 2020
[The influence on the BK channel beta1 subunit expression of SO caused by high cholesterol in rabbits]
Pang Du1, Jing-Guo Wei, Guang-Bin Cui
1Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. guojjj@fmmu.edu.cn
Aim:
To investigate the effects on protein expression of big-conductance Ca(2+)-sensitive K(+) channel (BKca) beta1 subunit caused by high cholesterol in Rabbit Oddi's sphincter (SO) cells.
Methods:
The rat-anti-rabbit polyclonal antiserum against beta1 subunits of BKca channel of SO cell was prepared. And the protein expression of BKca channel beta1 subunit of SO tissue was detected by semi-quantitative immunohistochemical staining.
Results:
The protein expression of BKca channels beta1 subunit of SO tissue in HC group was reduced, and there's statistically significant difference between the HC group and the control group.
Conclusion:
High cholesterol can reduce the protein expression of BK Channel's beta1 subunit in Rabbits' SO which suggests high cholesterol can affect the function of BKca channel.
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