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Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer
Ashok Agarwal1, Rakesh K Sharma
1Reproductive Research Center, Glickman Urological Institute, Cleveland, Ohio 44195, USA. agarwaa@ccf.org
Objective:
To evaluate the performance of the automated semen quality analyzer system for assessing sperm quality.
Design:
Double-blind prospective study.
Setting:
Tertiary care hospital.
Patient(S):
Fifty healthy men donated semen samples.
Intervention(S):
None.
Main Outcome Measure(S):
Precision, accuracy and agreement between automated and manual semen analysis methods was assessed for sperm concentration, motility, morphology, and known concentrations of latex bead quality control media.
Result(S):
A good agreement was seen between the results of sperm concentration reported by the SQA-V automated analyzer (Spermalite/SQA-V; Medical Electronic Systems Ltd, Caesarea Industrial Park, Israel) and those obtained manually. A similar linearity was seen when the SQA-V results were compared with the manual data and also when the manual results of individual operators were compared with each other. The automated assessment of morphology showed high sensitivity (89.9%) for identifying percent normal morphology, and the precision of the SQA-V was considerably higher when compared with the manual method. The interoperator variability for manual assessment was significant. The automated analysis was quick compared with the manual method.
Conclusion(S):
The SQA-V can be used interchangeably with manual semen analysis methods for examining sperm concentration and motility. The automated SQA-V analyzer is more precise and shows the ability to accurately classify normal versus abnormal sperm morphology.
