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Hepatocyte growth factor is a potent stimulator of human melanocyte DNA synthesis and growth
K Matsumoto1, H Tajima, T Nakamura
1Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Biochemical and Biophysical Research Communications
|April 15, 1991
Abstract:
Hepatocyte growth factor (HGF) is a potent mitogen for adult rat hepatocytes in primary culture. HGF stimulates growth and DNA synthesis of normal human epidermal melanocytes in culture. The maximal stimulation of DNA synthesis by 4.0-fold occurred with 10 ng/ml HGF. This stimulatory effect was additive with both acidic and basic fibroblast growth factors, while it was inhibited by transforming growth factor-beta 1. Melanocytes expressed a single class of specific, high-affinity receptors for HGF with a Kd of 22 pM and approximately 120 receptors/cell. Thus, HGF is a potent mitogen for normal human epidermal melanocytes.