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Response surface methodology for the evaluation of glucose-6-phosphate dehydrogenase enrichment process by soybean
Francislene Andréia Hasmann1, Daniela de Borba Gurpilhares, Inês Conceição Roberto
1Department of Biotechnology/Engineering School of Lorena/University of São Paulo, Rod. Itajubá-Lorena, Km 74.5, 12.600 - 000, P.O. Box 116, Lorena/SP, Brazil. francislene@debiq.faenquil.br
Abstract:
Glucose-6-phosphate dehydrogenase (G6PD) present in Saccahromyces cerevisiae is an enzyme of the pentose pathway. An effective enrichment of this intracellular enzyme can be achieved with the reversed micellar methodology. In this work, this methodology was employed with soybean lecithin, a biocompatible surfactant. A factorial design was used to evaluate the influence of pH (A) and extraction runs (B) on the G6PD purification factor. After statistical analysis and process optimization, a mathematical model representing G6PD enrichment was obtained: Y=4.89-0.83A+0.092B+0.27AB-1.37B2 with an enzyme purification factor of about 5.2.
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