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Updated: Jul 19, 2026
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Chemiluminescence of synephrine based on the cerium(IV)-rhodamine B system
Ruifen Wang1, Lijuan Wan, Qian Li
1College of Chemistry and Chemical Engineering, Institute of Analytical Sciences, Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Southwest University, Beibei, Chongqing 400715, People's Republic of China.
Abstract:
A new chemiluminescence (CL) method is described for the determination of synephrine. It is based on the reaction between synephrine and Ce(IV) in a nitric acid medium and measurement of the CL intensity produced by rhodamine B used as a luminophore, similar to luminol or lucigenin in basic media, instead of as a sensitizer. In the optimum conditions, the increase of CL intensity was correlated with synephrine concentration over the range 5.0 x 10(-9)-1.0 x 10(-6) g/mL with a detection limit of 1.0 x 10(-9) g/mL. The relative standard deviation (RSD) was 2.9% for 1.0 x 10(-7) g/mL synephrine (n = 11). The method was applied to the determination of a drug in herbal products, citrus fruit and biological samples, with satisfactory results. The results given by the proposed method are in good agreement with those given by HPLC-UV and UV spectrophotometry.
