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Related Concept Videos

Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
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MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Related Experiment Video

Updated: Jul 19, 2026

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Published on: April 7, 2012

Microbial metabolomics: past, present and future methodologies.

Mlawule R Mashego1, Karl Rumbold, Marjan De Mey

  • 1Department of Biotechnology, Faculty of Applied Sciences, Technical University of Delft, Delft, The Netherlands. M.Mashego@tnw.tudelft.nl

Biotechnology Letters
|November 9, 2006
PubMed
Summary

Microbial metabolomics enables comprehensive analysis of cellular metabolites. This review details experimental protocols for accurately quantifying microbial metabolites using advanced analytical techniques.

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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Last Updated: Jul 19, 2026

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
11:47

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Published on: April 7, 2012

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Area of Science:

  • Microbiology
  • Metabolomics
  • Analytical Chemistry

Background:

  • Microbial metabolomics is crucial for drug discovery and metabolic engineering.
  • Metabolomics involves comprehensive analysis of low molecular weight metabolites in cells.
  • Accurate quantification of microbial metabolites under specific conditions is essential.

Purpose of the Study:

  • To review the development of experimental protocols in microbial metabolomics.
  • To focus on methods for reliable quantification of microbial metabolites.
  • To discuss past, current, and future trends in the field.

Main Methods:

  • Rapid sample collection and metabolic activity quenching.
  • Intracellular metabolite extraction techniques.
  • Quantification using enzyme-based assays and hyphenated analytical techniques like LC-MS(n), GC-MS(n), CE-MS(n), and NMR spectroscopy.

Main Results:

  • Advancements in sample handling and metabolite extraction.
  • Integration of advanced analytical platforms for sensitive detection.
  • Improved reliability and accuracy in metabolite quantification.

Conclusions:

  • Continuous development of experimental protocols is vital for microbial metabolomics.
  • Hyphenated analytical techniques coupled with mass spectrometry and NMR are key.
  • Future research will likely focus on further refining quantification accuracy and throughput.