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Purification of a trypsin inhibitor from sweet potato in an aqueous two phase system
Kuen-Shan Jaw1, Lin Hung Chou, Shiao-Ming Chang
1Department of Bioengineering, Tatung University, Taipei, Taiwan.
Biotechnology Letters
|November 9, 2006
Abstract:
Trypsin inhibitor from sweet potato was extracted and purified in a single step using an aqueous two-phase system of polyethylene glycol 6000 (11% w/v), phosphate (16.5% w/v), KCl (9% w/v) and at pH 6. Purity of the trypsin inhibitor was enhanced 3.7-fold, and the recovery was 95%. The purified trypsin inhibitor showed one visible band, and the molecular size was 23 kDa by SDS-PAGE.

