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One-step homogeneous detection of DNA hybridization with gold nanoparticle probes by using a linear light-scattering

Bao-An Du1, Zheng-Ping Li, Cheng-Hui Liu

  • 1College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, P.R. China.

Angewandte Chemie (International Ed. in English)
|November 9, 2006
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...