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A simple assay for proteolysis of IGFBP-3
G Lamson1, L C Giudice, R G Rosenfeld
1Department of Pediatrics, Stanford University Medical Center, California 94305.
The Journal of Clinical Endocrinology and Metabolism
|June 1, 1991
Abstract:
An assay for assessing the proteolysis of IGFBP-3 by a biological fluid or protein has been developed using 125IhIGFBP-3 recombinantly derived from Escherichia coli as a substrate indicator. The labelled substrate is incubated at 37 degrees in the presence of the agent being tested and proteolysis is visualized by SDS-PAGE and autoradiography. The assay showed that the pregnancy associated protease is Ca++ dependent. The substrate is also proteolyzed by rat and mouse pregnancy sera, making the assay applicable to studies in those animal systems.