Mitochondrial DNA mutations and 8-hydroxy-2'-deoxyguanosine Content in Japanese patients with urinary bladder and

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Updated: Jul 18, 2026

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
14:12

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues

Published on: August 1, 2015

Takashi Wada1, Nozomu Tanji, Akira Ozawa

  • 1Department of Urology, Ehime University School of Medicine, Tohon-city, Ehime, Japan.

Anticancer Research
|November 11, 2006
PubMed
Abstract

Insights

Mitochondrial DNA (mtDNA) mutations were found in bladder and kidney cancers, but their presence was independent of oxidative damage markers like 8-hydroxy-2'-deoxyguanosine (8-OHdG). This suggests genetic instability in the mtDNA D-loop region of cancer tissues.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Mitochondrial DNA (mtDNA) mutations are increasingly found in human cancers.
  • Oxidative damage from reactive oxygen species (ROS) is a potential cause of these mutations.
  • Investigating the link between mtDNA mutations and ROS in cancer is crucial.

Purpose of the Study:

  • To examine mutations in the mtDNA D-loop region in urinary bladder and renal cancers.
  • To assess the levels of 8-hydroxy-2 -deoxyguanosine (8-OHdG) as a marker of oxidative damage in these cancers.
  • To determine the relationship between mtDNA mutations and 8-OHdG levels in cancer tissues.

Main Methods:

  • Direct sequencing of the mtDNA D-loop region in Japanese patients with bladder or renal cancer.
  • Measurement of 8-OHdG levels in excised tumor specimens from patients who underwent surgery.
  • Comparison of 8-OHdG levels in cancerous versus neighboring non-cancerous tissues.

Main Results:

  • Somatic mutations in the mtDNA D-loop were detected in 23% of bladder cancer patients and 14% of renal cancer patients.
  • The poly(C) repeat at positions 303-309 was the most common mutation site.
  • While 8-OHdG levels were higher in cancer tissues, many tumors with elevated 8-OHdG did not show D-loop mutations.

Conclusions:

  • The D-loop region of mtDNA exhibits genetic instability in cancer tissues.
  • This genetic instability appears to be independent of the 8-OHdG level.
  • Further research is needed to understand the mechanisms driving mtDNA D-loop mutations in cancer.

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