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Updated: Jul 18, 2026
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Published on: November 30, 2014
[Construction of suppression subtracted cDNA library of deltamethrin-resistant Aedes albopictus]
Jia-hong Wu1, Tong-yan Zhao, Yan-de Dong
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China.
Objective:
To construct the suppression subtracted cDNA library of deltamethrin-resistant Aedes albopictus.
Methods:
Total RNA was extracted from the deltamethrin-resistant (R-lab) and -sensitive (S-lab) isolates, mRNA was obtained after purification. Double stranded cDNAs were synthesized after reverse transcription. Two subtractions were performed by suppression subtractive hybridization with S-lab as tester and R-lab as driver or S-lab as driver and R-lab as tester. Enriched different expressed cDNA was cloned into pMD18-T vector to construct subtractive libraries.
Results:
The subtracted cDNA libraries contained 580 and 477 positive clones respectively. The PCR results of 150 clones picked randomly from each library showed that the positive ratio of constructed cDNA libraries was 93%, with a length of cDNA fragments ranged from 150bp to 750bp.
Conclusion:
The suppression subtracted cDNA library of deltamethrin-resistant Ae. albopictus is constructed.
