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Updated: Jan 1, 2026
Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay
Published on: September 30, 2021
[DNA amplification of Plasmodium vivax parasites from Giemsa-stained blood smears]
Fang-zhen Xiao1, Shan-ying Zhang, Long-shan Xu
1Fujian Provincial Center for Diseases Control and Prevention, Fuzhou, China.
Objective:
To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears.
Methods:
Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and identification of allelotype in the Plasmodium vivax merozoite surface protein-1 (PvMSP-1).
Results:
Target DNA bands appeared in all samples of unstained thick blood smears, while no DNA bands were visible in the fixed and stained thin smears. Both methods identified PvMSP-1 alleles from smears with parasitemia of > or = 0.01%.
Conclusion:
It is feasible to identify PvMSP-1 alleles from Giemsa-stained blood smear.
