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Published on: August 7, 2014
Biomarker discovery from body fluids using mass spectrometry.
Ana Villar-Garea1, Matthias Griese, Axel Imhof
1Histone modifications group, Adolf-Butenandt Institute, Ludwig-Maximilians University of Munich, Schillerstr 44, 80336 Munich, Germany.
Summary
Mass spectrometry (MS) offers a promising avenue for biomarker discovery in body fluids due to its simplicity. This review addresses challenges like reproducibility and statistical analysis to refine protocols for reliable biomarker identification.
Area of Science:
- Biomarker research
- Proteomics
- Analytical chemistry
Background:
- Systems analysis of body fluids using mass spectrometry (MS) is an emerging area for biomarker discovery.
- This technique is attractive due to minimal need for pre-identified biomarker candidates and straightforward sample preparation.
- However, early development faced challenges including poor reproducibility, blood-specific peptide characteristics, and inadequate statistical analysis.
Purpose of the Study:
- To review recent advancements in developing robust protocols for biomarker discovery from body fluids using mass spectrometry.
- To address and overcome the critical limitations previously identified in MS-based biomarker research.
Main Methods:
- Focus on systems analysis of non-digested blood serum and plasma samples.
- Utilized matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) and surface-enhanced laser desorption/ionization-time-of-flight (SELDI-TOF) mass spectrometry techniques.
Main Results:
- Significant efforts have been made to develop improved protocols for MS-based biomarker discovery.
- These advancements aim to enhance reproducibility and address the complexities of analyzing blood-derived peptides.
- Focus on appropriate statistical methodologies for data analysis is crucial.
Conclusions:
- Developing standardized and reproducible protocols is essential for the successful application of mass spectrometry in biomarker discovery from body fluids.
- Overcoming technical and analytical challenges will enable reliable identification of biomarkers in serum and plasma.
- This review highlights progress towards validated MS-based approaches for clinical diagnostics.
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