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Q-priming PCR: a quantitative real-time PCR system using a self-quenched BODIPY FL-labeled primer

Xiao Kun Liu1, Yan Hong

  • 1Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.

Analytical Biochemistry
|November 14, 2006
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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