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Biological markers for evaluation of root resorption
Laura Balducci1, Amsaveni Ramachandran, Jianjun Hao
1Department of Orthodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
Archives of Oral Biology
|November 14, 2006
Summary
Dentin sialoprotein (DSP) and phosphophoryn (PP) in gingival crevicular fluid can indicate root resorption during orthodontic treatment. Higher levels of these proteins correlate with more severe root loss.
Area of Science:
- Biochemistry
- Orthodontics
- Biomarkers
Background:
- External apical root resorption is a pathological process during orthodontic tooth movement.
- It involves the removal of cementum and dentin from the root surface under active force.
Purpose of the Study:
- To identify and quantify extracellular matrix proteins: dentin matrix protein 1 (DMP1), dentin phosphophoryn (PP), and dentin sialoprotein (DSP).
- To assess these proteins in gingival crevicular fluid (GCF) of subjects undergoing orthodontic treatment.
Main Methods:
- Subjects were categorized into mild (<2mm) and severe (>2mm) root resorption groups, plus a control group.
- Gingival crevicular fluid (GCF) was collected from upper incisors using filter paper strips.
- Proteins were analyzed using SDS-PAGE, Western blot, and ELISA; statistical analysis employed one-way ANOVA and Scheffé test.
Main Results:
- ELISA revealed significant differences in DMP1, PP, and DSP concentrations between control and root resorption groups.
- Concentrations of PP and DSP were significantly higher in the severe root resorption group compared to the mild group.
Conclusions:
- DSP and PP show potential as biological markers for monitoring root resorption during orthodontic treatment.
- Significant differences in these dentin-specific proteins were detected across all groups.