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Optisol corneal storage medium.

H E Kaufman1, R W Beuerman, T L Steinemann

  • 1Lions Eye Research Laboratories, LSU Eye Center, Louisiana State University, Medical Center School of Medicine, New Orleans 70112.

Archives of Ophthalmology (Chicago, Ill. : 1960)
|June 1, 1991
PubMed
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Optisol, a new corneal storage medium, better preserves endothelial cell morphology than Dexsol after 14 days of refrigeration. Both media are suitable for short-term room temperature storage.

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Cell Biology

Background:

  • Corneal storage media are crucial for preserving donor tissue viability.
  • Intermediate-term storage requires media that minimize endothelial cell damage.
  • Optisol and Dexsol are intermediate-term corneal storage media with different compositions.

Purpose of the Study:

  • To compare the efficacy of Optisol and Dexsol in preserving corneal endothelial cell morphology.
  • To evaluate the impact of storage temperature and duration on corneal structure.

Main Methods:

  • Scanning electron microscopy (SEM) was used to assess endothelial cell morphologic characteristics.
  • Corneas were stored in Optisol and Dexsol at 4°C for 14 days and at 26°C for 1-4 days.
  • Temperature-reversal analysis was performed to assess corneal thickness changes.

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Main Results:

  • Optisol-stored corneas exhibited significantly fewer morphologic changes compared to Dexsol-stored corneas after 14 days at 4°C.
  • No significant differences in morphology were observed between the two media after 1-4 days at 26°C.
  • Optisol-stored corneas remained significantly thinner than Dexsol-stored corneas throughout the study, with no significant thickness change upon warming after 2-week cold storage.

Conclusions:

  • Optisol demonstrates superior preservation of corneal endothelial cell morphology compared to Dexsol at refrigerator temperatures for up to 14 days.
  • Both Optisol and Dexsol may be suitable for short-term corneal preservation at room temperature, offering a safety margin during unreliable refrigeration.
  • Optisol's ability to maintain corneal thinness may be advantageous for specific clinical applications.