Related Experiment Video
Updated: Jul 18, 2026

Video-rate Scanning Confocal Microscopy and Microendoscopy
Published on: October 20, 2011
Reduction of the spherical aberration effect in high-numerical-aperture optical scanning instruments
Isabel Escobar1, Genaro Saavedra, Manuel Martínez-Corral
1Departmento de Optica, Universidad de Valencia, Burjassot, Spain.
Abstract:
In modern high-numerical-aperture (NA) optical scanning instruments, such as scanning microscopes, optical data storage systems, or laser trapping technology, the beam emerging from the high-NA objective focuses deeply through an interface between two media of different refractive index. Such a refractive index mismatch introduces an important amount of spherical aberration, which increases dynamically when scanning at increasing depths. This effect strongly degrades the instrument performance. Although in the past few years many different techniques have been reported to reduce the spherical aberration effect, no optimum solution has been found. Here we concentrate on a technique whose main feature is its simplicity. We refer to the use of purely absorbing beam-shaping elements, which with a minimum modification of optical architecture will allow a significant reduction of the spherical aberration effect. Specifically, we will show that an adequately designed reversed-Gaussian aperture permits the production of a focal spot whose form changes very slowly with the spherical aberration.

