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Updated: Jul 18, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Point sampling digital imagery with 'SamplePoint'.
D Terrance Booth1, Samuel E Cox, Robert D Berryman
1Rangeland Scientist and Remote Sensing Technician, USDA Agricultural Research Service, High Plains Grasslands Research Station, 8408 Hildreth Rd, Cheyenne, WY 82009, USA. Terry.Booth@ars.usda.gov
New software, SamplePoint, offers accurate and easy digital image analysis for measuring ground-cover occurrence. It rivals field methods, requiring minimal training for rapid, reliable data collection.
Area of Science:
- Ecological monitoring
- Remote sensing
- Image analysis
Background:
- Digital image analysis offers potential time and cost savings over traditional field measurements.
- Current image analysis methods often lack the accuracy of field measurements and require extensive user training.
- Accurate ground-cover measurement is crucial for ecological and environmental studies.
Purpose of the Study:
- To introduce SamplePoint, a novel software for digital image analysis.
- To evaluate the accuracy and ease of use of SamplePoint for ground-cover measurements.
- To provide a tool for calibrating image analysis software or direct measurement.
Main Methods:
- Development of SamplePoint software featuring a single-pixel sample point with context view.
- Comparative analysis of SamplePoint accuracy against established field measurement techniques.
- Assessment of user training requirements and measurement speed.
Main Results:
- SamplePoint achieved accuracy comparable to the most precise field-based ground-cover measurement methods.
- The software demonstrated ease of use, requiring minimal training for expert users.
- Rapid measurements from digital image data were facilitated by SamplePoint.
Conclusions:
- SamplePoint provides a highly accurate and efficient method for measuring percent occurrence from digital images.
- The software is suitable for both direct ground-cover measurements and for calibrating other image analysis tools.
- SamplePoint enhances the utility of digital imagery in ecological and environmental monitoring.
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