Related Experiment Video
Updated: Jun 28, 2026

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
Published on: July 11, 2025
Shadow attenuation with high dynamic range images. Creating RGB images that allow feature classification in areas
Samuel E Cox1, D Terrance Booth
1Agricultural Science Technician and Rangeland Scientist, USDA Agricultural Research Service, High Plains Grasslands Research Station, 8408 Hildreth Rd, Cheyenne, WY 82009, USA. Samuel.Cox@ars.usda.gov
Abstract:
Shadow often interferes with accurate image analysis. To mitigate shadow effects in near-earth imagery (2 m above ground level), we created high dynamic range (HDR) nadir images and used them to measure grassland ground cover. HDR composites were created by merging three differentially exposed images spanning a wide exposure range and resulted in lightened shadows. HDR images showed more detail; reduced the numbers of pure black, pure white, and pixels visually indistinguishable from black and white; reapportioned skewed luma values towards a normal distribution; and increased the Euclidean distance between litter and bare ground RGB values--allowing increased feature separation; all of which facilitated an increase in real feature classification through manual image analysis. Drawbacks to the method included decreased image sharpness due to minor misalignment of images or moving vegetation, time required to create HDR images, and difficulty with acquiring primary images from a moving platform. We conclude that HDR imagery can provide more accurate measurements of bare soil cover for ecosystem monitoring and assessment.
Related Concept Videos
Difference from Background: Limit of Detection
The LOD indicates the presence or absence...
Photoreceptors and Visual Pathways
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
