Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Pragmatic Randomized Trial Comparing Surgical Clipping and Endovascular Treatment of Unruptured Intracranial Aneurysms.

AJNR. American journal of neuroradiology·2023

Same author

The Magellanic Corona as the key to the formation of the Magellanic Stream.

Nature·2020

Same author

The potential of alcohol release doorplates to reduce surface contamination during hand contact.

The Journal of hospital infection·2017

Same author

Pilot study to determine whether microbial contamination levels in hospital washrooms are associated with hand-drying method.

The Journal of hospital infection·2017

Same author

Hydrogel versus Bare Platinum Coils in Patients with Large or Recurrent Aneurysms Prone to Recurrence after Endovascular Treatment: A Randomized Controlled Trial.

AJNR. American journal of neuroradiology·2017

Same author

Henry J.M. Barnett.

AJNR. American journal of neuroradiology·2016

Search research articles

Related Experiment Video

Updated: Jul 18, 2026

Detection and Isolation of Campylobacter spp. from Raw Meat

Detection and Isolation of Campylobacter spp. from Raw Meat

Published on: February 23, 2024

Specific detection of Campylobacter jejuni from faeces using single nucleotide polymorphisms.

E L Best¹, A J Fox, R J Owen

¹Laboratory of Enteric Pathogens, Centre for Infections, Health Protection Agency, London, UK. emma.best@hpa.org.uk

Epidemiology and Infection

|November 18, 2006

Summary

This study introduces a rapid real-time PCR method for detecting Campylobacter jejuni lineages directly from human faeces. The approach identifies specific single nucleotide polymorphisms (SNPs) to determine major clonal complexes, offering quick epidemiological data.

More Related Videos

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Published on: March 10, 2020

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Related Experiment Videos

Last Updated: Jul 18, 2026

Detection and Isolation of Campylobacter spp. from Raw Meat

Detection and Isolation of Campylobacter spp. from Raw Meat

Published on: February 23, 2024

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Published on: March 10, 2020

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)

Published on: October 30, 2016

Area of Science:

Microbiology and Molecular Diagnostics
Infectious Disease Epidemiology

Background:

Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide.
Traditional methods for C. jejuni typing, such as multi-locus sequence typing (MLST), are time-consuming and require isolates.
Rapid identification of C. jejuni lineages is crucial for timely epidemiological surveillance and public health interventions.

Purpose of the Study:

To develop and validate a rapid, direct molecular strategy for identifying C. jejuni clonal complexes from human faecal specimens.
To utilize single nucleotide polymorphisms (SNPs) associated with MLST clonal complexes for real-time PCR assay design.
To assess the accuracy of direct molecular identification against conventional MLST results.

Main Methods:

Analysis of C. jejuni MLST databases to identify lineage-specific SNPs.
Design and implementation of real-time PCR assays targeting six major C. jejuni clonal complexes.
Direct DNA extraction from human faecal specimens followed by real-time PCR, with confirmation by MLST on cultured isolates.

Main Results:

A rapid DNA extraction method allowed identification of 38% of isolates to a major MLST clonal complex directly from faeces in under 3 hours.
The real-time PCR assays successfully identified specific C. jejuni clonal complexes.
Results from the direct molecular method showed comparability with conventional MLST findings.

Conclusions:

A novel, rapid real-time PCR strategy based on SNPs enables direct detection and characterization of C. jejuni beyond species level from faecal samples.
This method provides real-time epidemiological data, offering a faster alternative to traditional typing methods.
The SNP-based approach demonstrates validity for direct molecular specimen identification and epidemiological surveillance.