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Updated: Jul 18, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Determination of hydroxycinnamic acids and volatile phenols in wort and beer by isocratic high-performance liquid
Nele Vanbeneden1, Filip Delvaux, Freddy R Delvaux
1Centre for Malting and Brewing Science, Department of Microbial and Molecular Systems - Food and Microbial Technology, K.U.Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium. nele.vanbeneden@biw.kuleuven.be
Abstract:
The suitability of a simple and rapid isocratic RP-HPLC method with amperometric electrochemical detection for the simultaneous detection and quantification of hydroxycinnamic acids and their corresponding aroma-active volatile phenols in wort and beer is reported. The technique gives good specificity and sensitivity, and can therefore be used for routine monitoring of hydroxycinnamic acids in wort and the development of volatile phenolic flavour compounds during the beer production process and subsequent conservation.
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