High-throughput measurements of biochemical responses using the plate::vision multimode 96 minilens array reader

Area of Science:

• Biotechnology
• High-Throughput Screening
• Drug Discovery

Background:

• High-throughput screening (HTS) is crucial for modern drug discovery.
• The plate::vision is a multimode reader for various detection methods.
• The plate::explorer is an ultrahigh-throughput screening system.

Purpose of the Study:

• To evaluate the integration of the plate::vision reader with the plate::explorer system for HTS.
• To assess the system's performance, throughput, and robustness in complex assays.
• To demonstrate the system's utility in accelerating the drug discovery pipeline.

Main Methods:

• Integration of the plate::vision multimode reader with the plate::explorer ultrahigh-throughput screening system.
• Utilizing event-driven software and parallel plate-handling for complex assays.
• Performing kinetic readouts in high-density microtiter plate formats.

Main Results:

• The plate::vision reader demonstrated performance comparable to other readers.
• The integrated system enabled the execution of complex assays with kinetic readouts.
• Over 30 million data points were generated over five years of use.

Conclusions:

• The combined plate::vision and plate::explorer system offers high throughput and robust performance for HTS.
• This integrated system significantly enhances the efficiency of the drug discovery process.
• The system facilitates the generation of large datasets essential for identifying drug candidates.