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Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Related Experiment Video

Updated: Jul 18, 2026

Studying Wnt Signaling During Patterning of Conducting Airways
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Published on: October 16, 2016

NPAS1 regulates branching morphogenesis in embryonic lung.

Bernadette M Levesque1, Shutang Zhou, Lin Shan

  • 1Department of Pathology, Duke University Medical Center, Research Drive, Carl Building, Room 0043, Durham, NC 27710, USA.

American Journal of Respiratory Cell and Molecular Biology
|November 18, 2006
PubMed
Summary

The npas1 gene is crucial for embryonic lung development in mice, regulating branching, cell differentiation, and myofibroblast formation. Its expression is vital for normal lung morphogenesis and neuroendocrine cell development.

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Last Updated: Jul 18, 2026

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Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
09:45

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The master regulator of tracheogenesis in Drosophila, trachealess (Trl), lacks a known functional mammalian homolog.
  • This suggests that similar genes may govern lung development in mammals.

Purpose of the Study:

  • To investigate the role of the npas1 gene in murine embryonic lung development.
  • To determine if npas1 is a functional homolog of Trl in regulating lung morphogenesis.

Main Methods:

  • Quantitative (Q)RT-PCR and immunostaining to analyze npas1 expression patterns.
  • Antisense oligodeoxynucleotides (ODN) to inhibit npas1 and arnt function.
  • Microarray analysis to identify downstream gene expression changes.
  • Immunostaining for alpha-smooth muscle actin and PGP9.5 to assess myofibroblast and neuroendocrine cell populations.

Main Results:

  • npas1 is expressed in the developing murine lung, peaking early (Embryonic Day 10.5-11.5) in parabronchial mesenchymal cells.
  • Inhibition of npas1 or arnt by antisense ODN impaired lung branching morphogenesis.
  • npas1 inhibition led to altered myofibroblast development and increased pulmonary neuroendocrine cells.
  • Microarray analysis revealed downregulation of over 50 genes involved in cell migration and differentiation, including neurogenic and muscle development genes.

Conclusions:

  • npas1 plays a significant role in regulating murine embryonic lung development.
  • npas1 influences branching morphogenesis, myofibroblast distribution, and neuroendocrine cell differentiation.
  • npas1 may function as a mammalian counterpart to Drosophila trachealess in regulating airway development.