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Updated: Jul 18, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
[Rapid identification of compounds in cortex moutan by liquid chromatography-tandem mass spectrometry]
Shun-jun Xu1, Liu Yang, Mian Zhang
1Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Aim:
To analyze the chemical constituents of cortex moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.
Methods:
An on-line optimized HPLC-DAD/MS2 technique was employed.
Results:
In the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated. Among them, over thirty compounds were identified, including paeonol, paeonilflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, galloyloxypaeoniflorin, mundanpioside A, C, D, E, H, etc. by the high performance liquid chromatography with diode-array detection in parallel with electrospray ionization and quadrupole-time of flight tandem mass spectrometry (HPLC-DAD/ESI-MS2).
Conclusion:
This method can be used to rapidly determine the constituents of cortex moutan.
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