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[Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method]
Ying-feng Zhang1, Li-ling Zhou, Rui Li
1Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China. fredzhangfeng@163.com
Aim:
To determine in vitro the rat plasma protein binding rate by using microdialysis method.
Methods:
The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation: f = ( C0 - Cf)/C0.
Result:
The binding rate was kept relatively stable in the studied concentration range.
Conclusion:
It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
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