Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reducing noise associated with restraint box operation can facilitate animal handling in cattle abattoirs.

New Zealand veterinary journal·2025
Same author

Remembering Alexander S. Flockhart.

British dental journal·2025
Same author

Corrigendum to "A comparison of the efficacy of trastuzumab deruxtecan in advanced HER2-positive breast cancer: active brain metastasis versus progressive extracranial disease alone": [ESMO Open 8 (2023) 102033].

ESMO open·2024
Same author

A comparison of the efficacy of trastuzumab deruxtecan in advanced HER2-positive breast cancer: active brain metastasis versus progressive extracranial disease alone.

ESMO open·2023
Same author

Sample size determination for external pilot cluster randomised trials with binary feasibility outcomes: a tutorial.

Pilot and feasibility studies·2023
Same author

First Observation of the β3αp Decay of ^{13}O via β-Delayed Charged-Particle Spectroscopy.

Physical review letters·2023

Related Experiment Video

Updated: Jul 18, 2026

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Convective flow effects on DNA biosensors.

J Bishop1, S Blair, A Chagovetz

  • 1Department of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT, USA.

Biosensors & Bioelectronics
|November 23, 2006
PubMed
Summary

Convective flux enhances biosensing by influencing DNA hybridization. Lower affinity species control enhancement magnitude, while higher affinity species determine the upper limit, considering competitive binding.

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: Jul 18, 2026

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Analytical Chemistry

Background:

  • Biosensing systems rely on detecting target species through binding events.
  • Mass transport limitations can affect the sensitivity and speed of biosensors.
  • Convective flux offers a potential mechanism to enhance analyte delivery to sensing surfaces.

Purpose of the Study:

  • To investigate the theoretical limits of convective flux effects on biosensing performance.
  • To analyze how species concentrations and sample volumes influence convective enhancements.
  • To understand the role of differing affinities and competitive binding in these enhancements.

Main Methods:

  • Utilized a finite element method (FEM) to simulate mass transport of DNA in a microfluidic chamber.
  • Modeled DNA hybridization kinetics at the sensing surface.
  • Analyzed the interplay between convective flux, species concentrations, sample volumes, and binding affinities.

Main Results:

  • Convective flux enhancements are primarily governed by the concentration and sample volume of the lower affinity species.
  • The upper limit of these enhancements is dictated by the concentration and sample volume of the higher affinity species.
  • The degree of competition between species, linked to binding site occupancy, significantly controls enhancement levels.

Conclusions:

  • Convective flux can significantly enhance biosensing capabilities, particularly in microfluidic systems.
  • Optimizing convective transport requires careful consideration of both lower and higher affinity species' properties.
  • Understanding competitive binding dynamics is crucial for maximizing biosensor performance through flow control.