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Updated: Jul 18, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Nitrogen removal from wastewater using simultaneous nitrate reduction and anaerobic ammonium oxidation in single
Tatsuo Sumino1, Kazuichi Isaka, Hajime Ikuta
1Matsudo Research Laboratory, Hitachi Plant Technologies, 537 Kami-Hongo, Matsudo-shi, Chiba 271-0064, Japan. tatsuo.sumino.cj@hitachi-pt.com
Abstract:
The effects of C/N ratio and total organic carbon (TOC) loading on nitrogen removal through simultaneous nitrate reduction and anaerobic ammonium oxidation in a single reactor were examined. Granular sludge taken from a methane fermentation reactor was placed in an upflow reactor and supplied with synthetic wastewater containing nitrate at a C/N ratio of 1 to grow heterotrophic denitrifying bacteria. When nitrogen removal ratio reached 30%, anammox sludge attached to nonwoven-carrier was added into the same reactor and then ammonia was added to the synthetic wastewater. Nitrogen removal ratio was markedly increased to 80-94%. In this system, nitrogen removal ratio was affected by C/N ratio and TOC loading, not by the amount of granular sludge. A stable isotopic analysis using 15N-labeled nitrate showed that N2 gas was formed by anammox reaction.
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