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Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

Meiosis I

Meiosis I

Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
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Nondisjunction

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In Vitro Fertilization

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Related Experiment Video

Updated: Jul 16, 2026

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Methodological issues in infertility research.

¹ISIS Regional Fertility Centre, Mississauga, Ontario, Canada. dayas@mcmaster.ca

Best Practice & Research. Clinical Obstetrics & Gynaecology

|November 23, 2006

Summary

High-quality research is crucial for evidence-based healthcare decisions. This guide details ten methodological issues to improve the evaluation of therapeutic interventions, especially in infertility management.

Area of Science:

Clinical Epidemiology
Evidence-Based Medicine
Reproductive Medicine

Background:

Clinical decision-making relies on valid study evidence.
Appraising evidence quality is essential for effective healthcare.
Many studies have methodological limitations impacting reliability.

Purpose of the Study:

To discuss critical methodological issues in evaluating therapeutic intervention efficacy.
To provide a framework for informing gynecological practice, particularly in infertility management.
To enhance the quality of evidence used in clinical decision-making.

Main Methods:

Discussion of ten key methodological issues in study design and analysis.
Illustrative examples from medical literature.

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Related Experiment Videos

Last Updated: Jul 16, 2026

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model

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Published on: April 7, 2023

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization

Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization

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Focus on issues relevant to assisted reproductive technology and infertility.

Main Results:

Identified ten critical factors for rigorous study evaluation.
Emphasized the importance of randomization, blinding, and sample size.
Highlighted the need for appropriate analysis (e.g., intention-to-treat) and study orientation (superiority, equivalence).

Conclusions:

Rigorous methodological evaluation is paramount for reliable evidence in healthcare.
Adherence to these principles improves the quality of research informing clinical practice.
This approach is particularly relevant for optimizing infertility treatments.