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Updated: Jul 18, 2026

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
Published on: March 29, 2018
Increased type III and V collagen expression in human corpora lutea in early pregnancy
Masaaki Iwahashi1, Yasuteru Muragaki, Akira Ooshima
1Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama, Japan. masaaki@wakayama-med.ac.jp <masaaki@wakayama-med.ac.jp>
Objective:
To investigate the possible involvement of collagen in the characteristic structure and function of human corpora lutea (CL), type V collagen expression was determined in the CL tissues during the menstrual cycle and early pregnancy.
Design:
In vitro experiment.
Setting:
Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama, Japan.
Patient(S):
Regulatory cycling women and pregnant women with ovarian tumor and ectopic pregnancy who underwent adnexectomy.
Intervention(S):
Composition of the various types of collagen in human CL was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Main Outcome Measure(S):
Expression of type V collagen.
Result(S):
The ratios of type III to type I collagen and the ratios of type V to type I collagen in the CL tissues were significantly increased in early pregnancy compared with those in the menstrual cycle.
Conclusion(S):
These results suggest that alterations in composition of collagen might play an important role in determining the physiology and structure of the CL during the menstrual cycle and early pregnancy.
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