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Fast isolation of recombinant baculovirus by antibody screening
1Department of Chemistry, Max-Planck-Institute for Experimental Medicine, Göttingen, Germany.
Biotechniques
|February 1, 1991
Abstract:
The use of a rapid and efficient scheme for the detection and purification of recombinant baculoviruses is described. The method is based on the detection of foreign proteins in cellular lysates of baculovirus-infected insect cells by antibody screening. The recombinant virus is purified by repeated serial dilutions. The method allows the identification and purification of recombinant viruses within two to three weeks.