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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Chemiluminescent detection of DNA hybridization using gold nanoparticles as labels
Zheng-Ping Li1, Cheng-Hui Liu, Yong-Shan Fan
1College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, Hebei, China. lzpbd@mail.hbu.edu.cn
Abstract:
A chemiluminescent (CL) detection method has been developed for DNA hybridization. The assay relies on a sandwich-type DNA hybridization in which gold nanoparticles modified with alkylthiol-capped oligonucleotide strands are used as probes to monitor the presence of the specific target DNA. The AuCl(4)(-), which is the dissolving product of the gold nanoparticles anchored on the DNA hybrids, serves as an analyte in the H(2)O(2)-luminol- AuCl(4)(-) CL reaction for the indirect measurement of the target DNA. The combination of the remarkable sensitivity of the CL analysis with the large number of AuCl(4)(-) released from each DNA hybrid allows a detection limit at levels as low as 0.1 pM of the target DNA. Moreover, with a further silver amplification step, the detection limit will be pushed down to the femtomolar domain.
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