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Updated: Jul 18, 2026

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
Published on: August 22, 2018
Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary
Alexandra R Stettler1, Valérie Chaurin, Edwin C Constable
1Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland.
Abstract:
The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca(2+), Mg(2+), Mn(2+) and Ni(2+)) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.
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